Sensitivity Test of Pedada (Sonneratia alba) Leaf Extract Against the Growth of Staphylococcus aureus, Escherichia coli, and Aeromonas hydrophila Bacteria
DOI:
https://doi.org/10.31258/jocos.6.2.114-118Keywords:
Pathogenic Bacteria, Sonneratia alba, Sensitivity TestAbstract
Sonneratia alba (Pedada) is one of the potential mangroves as a natural antibacterial because it contains secondary metabolites, such as flavonoids, saponins, steroids, tannins, and terpenoids. This study aimed to determine the inhibitory power of Pedada leaf extract in inhibiting the growth of Staphylococcus aureus, Escherichia coli, and Aeromonas hydrophila. The research method used is experimental, including sample preparation, extraction, and sensitivity tests using the Kirby-Bauer disc method. This study used 100%, 50%, 25%, and 12.5% extract doses, positive control (Oxytetracycline), and negative control (a quade). The results showed that Pedada leaf extract could inhibit the growth of pathogenic bacteria; 100% concentration was the concentration with the widest inhibition zone diameter of S. aureus 15.48 mm, E. coli 13.37 mm, and A. hydrophila 13.88 mm. Based on this study's results, the best extract concentration for inhibiting bacterial growth is 100% concentration
Downloads
References
Aisyah, A., & Mulyani, Y. (2024). Peran Kloning Gen dalam Pengembangan Vaksin Rekombinan untuk Pencegahan Penyakit pada Ikan. Biosense, 7(02): 303–317.
Dotulong, V., Wonggo, D., & Montolalau, L.A.D.Y. (2018). Phytochemical Content, Total Phenols, and Antioxidant Activity of Mangrove Sonneratia alba Young Leaf Through Different Extraction Methods and Solvents. International Journal of ChemTech Research, 11(11): 356–363.
Fitri, N.W., & Rahayu, D. (2020). Aktivitas Antibakteri Ekstrak Tumbuhan Melastomataceae terhadap Bakteri Escherichia coli dan Staphylococcus aureus. Farmaka, 16(2): 69–77.
Ibrahim, Y.M., Dotulong, V., Wonggo, D., Lohoo, H.J., Montolalu, R.I., Makapedua, D.M. & Sanger, G. (2019). Aktivitas Antibakteri Infusa Daun Muda Mangrove Sonneratia alba Kering. Media Teknologi Hasil Perikanan, 7(2) : 52–57.
Kulkarni, C.P., & Manohara, S.M. (2024). Tinjauan Botani, Fitokimia dan Farmakologi Apel Bakau Sonneratia alba. Ilmu Farmasi Terapan, 14(5):1–11.
Kurniawan, R., Wahyuni, S., & Armando, F. (2022). Inhibition Test of Hot Water Extract of Rhizophora apiculata Leaves Against Edwardsiella tarda Bacteria in Vitro. Jurnal Natur Indonesia, 20(1): 30–34.
Langi, J.H., Wonggo, D., Damongilala, L.J., Montolalu, L.A.D.Y., Harikedua, S.D., & Makapedua, D.M. (2022). Flavonoid dan Tanin Ekstrak Air Subkritis Benang Sari Dan Kepala Putik Bunga Mangrove Sonneratia alba. Media Teknologi Hasil Perikanan, 10(3):157.
Liao, C.Y., Balasubramanian, B., Peng, J.J., Tao, S.R., Liu, W.C., & Ma, Y. (2021). Antimicrobial Resistance of Escherichia coli from Aquaculture Farms and Their Environment in Zhanjiang, China. Frontiers in Veterinary Science, 8: 806653.
Lingga, A.R., Pato, U., & Rossi, E. (2016). Uji Antibakteri Ekstrak Batang Kecombrang (Nicolaia speciosa Horan) terhadap Staphylococcus aureus dan Escherichia coli. Universitas Riau.
Linggama, G.A., Montolalu, L.A., Salindeho, N., Taher, N., Harikedua, S.D., Makapedua, D.M. & Damongilala, L.J. (2019). Aktivitas Antibakteri Ekstrak Air Rebusan Daun Mangrove Segar Sonneratia alba di Desa Wori Kabupaten Minahasa Utara. Media Teknologi Hasil Perikanan, 7(2): 41–45.
Manuhuttu, D., & Saimima, N.A. (2021). Potensi Ekstrak Daun Mangrove (Sonneratia alba) sebagai Antibakteri terhadap Salmonella, Staphylococcus aureus, dan Escherichia coli. Biologi Pendidikan dan Terapan, 7(2):71.
Nusaibah, N., Sari, R.M. & Widianto, D.I. (2022). Pemanfaatan Ekstrak Daun Pedada (Sonneratia caseolaris) dan Daun Katang-Katang (Ipomoea pes-caprae) sebagai Agen Antioksidan pada Formulasi Face Mist. Jurnal Pengolahan Hasil Perikanan Indonesia, 25(3): 441–456.
Purba, P.Y., Yoswaty, D., & Nursyirwani, N. (2022). Antibacterial Activity of Avicennia alba Leaves and Stem Extracts Against Pathogenic Bacteria (Pseudomonas aeruginosa, Aeromonas salmonicida, Staphylococcus aureus). Journal of Coastal and Ocean Sciences, 3(2): 144–151.
Rahayuningsih, S.R., Patimah, S.S., Mayanti, T., & Rustama, M.M. (2023). Aktivitas Antibakteri Ekstrak n-Heksana Daun Mangrove (Rhizospora stylosa Griff) terhadap Bakteri Patogen pada Ikan Nila (Oreochromis niloticus). Journal of Marine Research, 12(1): 1–6.
Ramadhani, A., Sari, N.I. & Sidauruk, S.W. (2022). Aktivitas Antibakteri Gram Positif Bacillus cereus dari Daun Mangrove (Sonneratia alba) Muda. Marinade, 5(02):149–156.
Rina, O., Chaniago, A.D., Suroso, E., Purnomo, A., & Sofiyan, A. (2024). Identifikasi Mangrove Sebagai Sumber Antioksidan di Ecowisata Cuku Nyinyi Desa Sidodadi Pesawaran Provinsi Lampung. JCI Jurnal Cakrawala Ilmiah, 3(11) : 2965–2976.
Sinubu, W.V., Tumbol, R.A., Undap, S.L., Manoppo, H. & Kreckhoff, R.L. (2022). Identifikasi Bakteri Patogen Aeromonas sp. pada Ikan Nila (Oreochromis niloticus) di Desa Matungkas, Kecamatan Dimembe, Kabupaten Minahasa Utara. Budidaya Perairan, 10(2): 109–120.
Sinurat, A.A.P., Renta, P.P., Herliany, N.E., & Purnama, D. (2019). Uji Aktivitas Antibakteri Ekstrak Metanol Rumput Laut Gracilaria edulis terhadap Bakteri Aeromonas hydrophila. Jurnal Enggano, 4(1): 105–114.
Sugiaman, V.K., Viando, E.J., & Pranata, N. (2023). Aktivitas Antibakteri Ekstrak Daun Mangga Gedong terhadap Streptococcus mutans. Jurnal Kedokteran Gigi Universitas Padjadjaran, 35(2): 134.
Ulfah, M. (2020). Aktivitas Antibakteri Ekstrak Aseton Rimpang Kunyit (Curcuma domestica) terhadap Bakteri Staphylococcus aureus dan Escherichia coli. Farmasi Muhamadiyah Kuningan, 5(1): 25–31.
Vaiyapuri, M., Joseph, T.C., Rao, B.M., Lalitha, K.V., & Prasad, M.M. (2019). Methicillin‐Resistant Staphylococcus aureus in Seafood Prevalence, Laboratory Detection, Clonal Nature, and Control in Seafood Chain. Journal of Food Science, 84(12): 3341–3351.
Yudha, K.C., Ibrahim, M., & Trimulyono, G. (2013). Aktivitas Antibakteri Ekstrak Herba Krokot (Portulaca oleracea L.) terhadap Staphylococcus aureus dan Escherichia coli. LenteraBio, 2(1): 87–93.
Downloads
Published
Issue
Section
License
Copyright (c) 2025 Siti Ada Wiya, Sri Wahyuni, Asri Mursawal, Nursyirwani Nursyirwani (Author)
This work is licensed under a Creative Commons Attribution 4.0 International License.
